Journal: Advanced Science

Article Title: A Frog Skin‐Derived Peptide Targeting SCD1 Exerts Radioprotective Effects Against Skin Injury by Inhibiting STING‐Mediated Inflammation

doi: 10.1002/advs.202306253

Figure Lengend Snippet: Macropinocytosis‐mediated uptake of radiation‐induced frog skin peptide 2 (RIFSP‐2) protects skin cells against radiation‐induced injury. A) Administration of FITC‐labeled RIFSPs to HaCaT cells and identification of the membrane impermeability of RIFSP‐2 through confocal microscopy. B) Time‐ and dose‐dependent uptake of FITC‐RIFSP‐2 by HaCaT cells detected through flow cytometry analysis. C) Influence of heparin, amiloride, and cytochalasin D on the uptake of FITC‐RIFSP‐2 by HaCaT cells through confocal microscopy detection. The effect of RIFSP‐2 on D) cell proliferation, E) cell cycle, and F,G) senescence, as detected by colony formation assay, propidium iodide (PI) staining and SA‐β‐Gal staining analysis, respectively. H) Colocalization analysis showing FITC‐RIFSP‐2 (green) and Mito‐Tracker (red) in irradiated HaCaT and WS1 cells. I–L) Influence of RIFSP‐2 on cellular ROS accumulation, mitochondrial ROS accumulation, energy production, and mitochondrial membrane potential in irradiated WS1 cells through DCFH‐DA staining, MitoSox probe staining, ADP probe staining and JC‐1staining, respectively. M) Effect of RIFSP‐2 on the homeostasis of actin filaments in irradiated skin cells, as detected by rhodamine phalloidin and G‐actin staining assays. N) The actin polymerization inhibitor, cytochalasin B (CytB, 20µ m , 2h), and the actin depolymerization agonist, 5a‐Pregnane‐3,20‐dione (5αP, 100n m , 24h) were administrated before RIFSP‐2 treatment. Investigation of cell death in irradiated skin cells was adopted with AV/PI staining kits. O) and P) Calcein AM release assay for understanding the influence of RIFSP‐2 on lytic cell death in irradiated HaCaT cells. * P < 0.05 and ** P < 0.01, compared with the control group.

Article Snippet: The WS1 cell line (human skin fibroblasts) was purchased from ATCC.

Techniques: Labeling, Membrane, Confocal Microscopy, Flow Cytometry, Colony Assay, Staining, Irradiation, Release Assay, Control

Journal: Advanced Healthcare Materials

Article Title: Cyclic Stretching Triggers Cell Orientation and Extracellular Matrix Remodeling in a Periodontal Ligament 3D In Vitro Model

doi: 10.1002/adhm.202301422

Figure Lengend Snippet: Biocompatibility evaluation. a) PDLF cells were cultivated within different conditions of hydrogel for up to 21 days. The cell viability was tested via Live/Dead staining (Green: Calcein‐AM, Red: Ethidium homodimer‐1, Blue: Hoechst 33258, scale bar: 200 µm) and b) quantified the percentage of alive PDLF cells at different time points by one‐way ANOVA ( n = 3, ns: no significant difference, **: p < 0.01, ****: p < 0.001). Abbreviations: Alg—Alginate; AC—Alginate/Collagen; AG—Alginate/Gelatin; AGC—Alginate/Gelatin/Collagen.

Article Snippet: Human periodontal ligament fibroblasts (PDLF; #CC‐7049 Lonza, Cologne, Germany) was expanded in a monolayer in high glucose Dulbecco's Modified Eagle Medium (DMEM; Sigma Aldrich, Taufkirchen, Germany) supplemented with 1% penicillin/streptomycin (Thermo Fisher Scientific, USA), 2% l ‐Glutamine (Sigma‐Aldrich, USA), 50 mg L −1 ascorbic acid (Sigma‐Aldrich), and 10% fetal calf serum (FCS, PAN Biotech, Germany).

Techniques: Staining